As science becomes ever more collaborative, the mechanisms for working in large and more diverse groups become more necessary. In the present article, I explore the utility of within-group collaboration agreements on participant conduct toward other project participants, within-group data sharing, and authorship of published manuscripts for research groups. Such agreements can solidify the expectations of the interactions among collaborators, potential rewards, and a feeling of security for those involved in the projects. They could also lead to more productive and satisfying research, as well as improving the training of future scientists.
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Abstract The grassland biome is an important sink for atmospheric methane (CH4), a major greenhouse gas. There is considerable uncertainty in the grassland CH4sink capacity due to diverse environmental gradients in which grasslands occur, and many environmental conditions can affect abiotic (e.g., CH4diffusivity into soils) and biotic (e.g., methanotrophy) factors that determine spatial and temporal CH4dynamics. We investigated the relative importance of a soil's gas diffusivity versus net methanotroph activity in 22 field plots in seven sites distributed across the US Great Plains by making approximately biweekly measures during the growing seasons over 3 years. We quantified net methanotroph activity and diffusivity by using an approach combining a gas tracer, chamber headspace measurements, and a mathematical model. At each plot, we also measured environmental characteristics, including water‐filled pore space (WFPS), soil temperature, and inorganic nitrogen contents, and examined the relative importance of these for controlling diffusivity and net methanotroph activity. At most of the plots across the seven sites, CH4uptake rates were consistently greatest when WFPS was intermediate at the plot level. Our results show that variation in net methanotroph activity was more important than diffusivity in explaining temporal variations in net CH4uptake, but the two factors were equally important for driving spatial variation across the seven sites. WFPS was a significant predictor for diffusivity only in plots with sandy soils. WFPS was the most important control on net methanotroph activity, with net methanotroph activity showing a parabolic response to WFPS (concave down), and the shape of this response differed significantly among sites. Moreover, we found that the WFPS level at peak net methanotroph activity was strongly correlated with the mean annual precipitation of the site. These results suggest that the local precipitation regime determines unique sensitivity of CH4uptake rates to soil moisture. Our findings indicate that grassland CH4uptake may be predicted using local soil water conditions. More variable soil moisture, potentially induced through predicted future extremes of rainfall and drought, could reduce grassland CH4sink capacity in the future.
Free, publicly-accessible full text available September 1, 2025 -
Abstract Nitrogen (N) availability is a well‐known driver of ecosystem structure and function, but as air quality regulations continue to reduce atmospheric N deposition, there is a need to understand how managed and unmanaged ecosystems respond to widespread decreases in terrestrial N availability. Historical N eutrophication, from pollution or fertilisation, may continue to constrain contemporary responses to decreases in available N because of altered plant and microbial feedbacks. Thus, while certain management practices like prescribed fire remove N from grassland ecosystems, the role of fire supporting ecosystems recovering from chronic N input is unknown.
To address this knowledge gap, we ceased a 30‐year N‐fertilisation treatment at a field experiment in a tallgrass prairie ecosystem crossed with burned and fire‐suppressed (unburned) treatments. We established subplots within each previously fertilised, recovering plot, fertilised at the same historical rate (10 g N m−2 year−1as NH4NO3), to compare plant and soil properties in recovering plots with control (never‐fertilised) and still‐fertilised treatments within different fire regimes.
We document different N‐fertilisation legacies among ecosystem properties in burned and unburned prairies recovering from N‐fertilisation. Soil N availability, nitrification and denitrification potentials in recovering plots remained higher than controls for 3–5 years—indicative of positive legacies—in both burned and unburned prairies, but burning did not reduce this legacy. In burned prairies, however, a positive legacy in above‐ground plant production persisted because a more productive grass species (switchgrass) replaced the previously dominant species (big bluestem) even though root C:N, but not soil C:N, increased to return back to control levels. Consequently, the main N loss pathways in burned and unburned prairies (pyrovolatilisation and microbially mediated processes, respectively) led to similar losses of soil total N (20–28 g N m−2) over 5 years.
Synthesis : Our results indicate that N eutrophication induces positive legacies of ecosystem functions that can persist for at least half a decade. N‐induced legacies arise because of shifts in soil microbial N‐cycling and plant functional traits. As a result, different management practices may elicit similar trajectories of ecosystem recovery in terms of total and available soil N because of different plant and microbial feedbacks.Free, publicly-accessible full text available September 1, 2025 -
Abstract Bison have long been considered a keystone species of North American prairies, increasing plant and animal diversity through a number of unique behaviors. One such behavior is wallowing, in which the repeated rolling of bison in the same spot leads to the formation of small, shallow, oval depressions called wallows. The objective of this study was to characterize spatial and physical attributes of bison wallows at the Konza Prairie Biological Station, a tallgrass prairie preserve in northeastern Kansas. We used aerial imagery from two different years (2011 and 2019) to assess the abundance and spatial distribution of these wallows in relation to fire frequency, elevation, and slope. We also recorded physical characteristics (2020) for a randomly selected subset of wallows and analyzed these data in relation to the same landscape features. Results from the analysis of the aerial images indicated that wallows were more abundant in areas characterized by combinations of more frequent burning, higher elevations, and little or no slope. In the 2020 physical measurements, we found that wallows were smaller in areas burned more often and shallower at higher elevations, particularly when located on grazing lawns. Terrestrial plants were found in approximately 72.1% of the wallows that were physically sampled, and their prevalence increased with increasing slope. We found some quantity of aquatic plants in approximately 7.1% of the sampled wallows. The probability of finding aquatic vegetation in wallows was higher on grazing lawns and in areas burned less frequently, particularly every 20 years. This study enhances the understanding of the distribution of wallows and their physical characteristics as a type of disturbance that could alter relationships within grassland communities.
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Abstract Background and aims A synergistic response of aboveground plant biomass production to combined nitrogen (N) and phosphorus (P) addition has been observed in many ecosystems, but the underlying mechanisms and their relative importance are not well known. We aimed at evaluating several mechanisms that could potentially cause the synergistic growth response, such as changes in plant biomass allocation, increased N and P uptake by plants, and enhanced ecosystem nutrient retention.
Methods We studied five grasslands located in Europe and the USA that are subjected to an element addition experiment composed of four treatments: control (no element addition), N addition, P addition, combined NP addition.
Results Combined NP addition increased the total plant N stocks by 1.47 times compared to the N treatment, while total plant P stocks were 1.62 times higher in NP than in single P addition. Further, higher N uptake by plants in response to combined NP addition was associated with reduced N losses from the soil (evaluated based on soil δ15N) compared to N addition alone, indicating a higher ecosystem N retention. In contrast, the synergistic growth response was not associated with significant changes in plant resource allocation.
Conclusions Our results demonstrate that the commonly observed synergistic effect of NP addition on aboveground biomass production in grasslands is caused by enhanced N uptake compared to single N addition, and increased P uptake compared to single P addition, which is associated with a higher N and P retention in the ecosystem.
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Abstract Hyperspectral remote sensing has the potential to map numerous attributes of the Earth’s surface, including spatial patterns of biological diversity. Grasslands are one of the largest biomes on Earth. Accurate mapping of grassland biodiversity relies on spectral discrimination of endmembers of species or plant functional types. We focused on spectral separation of grass lineages that dominate global grassy biomes: Andropogoneae (C4), Chloridoideae (C4), and Pooideae (C3). We examined leaf reflectance spectra (350–2,500 nm) from 43 grass species representing these grass lineages from four representative grassland sites in the Great Plains region of North America. We assessed the utility of leaf reflectance data for classification of grass species into three major lineages and by collection site. Classifications had very high accuracy (94%) that were robust to site differences in species and environment. We also show an information loss using multispectral sensors, that is, classification accuracy of grass lineages using spectral bands provided by current multispectral satellites is much lower (accuracy of 85.2% and 61.3% using Sentinel 2 and Landsat 8 bands, respectively). Our results suggest that hyperspectral data have an exciting potential for mapping grass functional types as informed by phylogeny. Leaf‐level hyperspectral separability of grass lineages is consistent with the potential increase in biodiversity and functional information content from the next generation of satellite‐based spectrometers.
Free, publicly-accessible full text available February 1, 2025 -
Abstract Many savannas are experiencing increased cover of trees and shrubs, resulting in reduced herbaceous productivity, shifts in savanna functional structure and potential reductions in ecotourism. Clearing woody plants has been suggested as an effective management strategy to mitigate these effects and restore these systems to an open state with higher rates of grass production and herbivory. This study investigated the effectiveness of repeated shrub clearing as a tool to mitigate bush encroachment in a semi‐arid savanna in southern Africa.
We present data from a 7‐year experiment in the Mthimkhulu Game Reserve bordering Kruger National Park, South Africa.
Colophospermum mopane stems and resprouting shoots were basally cut 2–3 times per year (2015–2022) in three pairs of treatment and control plots of 60 × 60 m. We monitored changes in soil moisture, grass biomass and herbivore activity via dung counts. We assessedC. mopane physiological responses to repeated cutting using non‐structural carbohydrates and stable water isotopes to infer changes to energy storage and functional rooting depth, respectively.The cleared treatment had higher soil moisture and grass biomass than the control treatment. Dung counts showed impala and buffalo visited the cleared treatment more frequently than the control treatment.
Repeated cutting had limited effects on
C. mopane survival in the first 2–3 years after initial clearing, but 80% of individuals were dead after 7 years. Repeatedly cutC. mopane had lower belowground starch concentrations and used water from shallower soil depths thanC. mopane in control plots.Synthesis and applications . Repeated cutting increased soil moisture availability and grass biomass, and attracted charismatic grazing herbivores. While more costly than once‐off clearing methods, this practice created more employment opportunities for a neighbouring rural community. Transforming portions of the ecosystem to a grass‐dominated state may increase ecotourism potential through improved game viewing in open systems. -
Abstract Plant traits can be helpful for understanding grassland ecosystem responses to climate extremes, such as severe drought. However, intercontinental comparisons of how drought affects plant functional traits and ecosystem functioning are rare. The Extreme Drought in Grasslands experiment (EDGE) was established across the major grassland types in East Asia and North America (six sites on each continent) to measure variability in grassland ecosystem sensitivity to extreme, prolonged drought. At all sites, we quantified community‐weighted mean functional composition and functional diversity of two leaf economic traits, specific leaf area and leaf nitrogen content, in response to drought. We found that experimental drought significantly increased community‐weighted means of specific leaf area and leaf nitrogen content at all North American sites and at the wetter East Asian sites, but drought decreased community‐weighted means of these traits at moderate to dry East Asian sites. Drought significantly decreased functional richness but increased functional evenness and dispersion at most East Asian and North American sites. Ecosystem drought sensitivity (percentage reduction in aboveground net primary productivity) positively correlated with community‐weighted means of specific leaf area and leaf nitrogen content and negatively correlated with functional diversity (i.e., richness) on an intercontinental scale, but results differed within regions. These findings highlight both broad generalities but also unique responses to drought of community‐weighted trait means as well as their functional diversity across grassland ecosystems.
Free, publicly-accessible full text available February 1, 2025 -
Abstract Shrub encroachment is one of the primary threats to mesic grasslands around the world. This dramatic shift in plant cover has the potential to alter ecosystem‐scale water budgets and responses to novel rainfall regimes. Understanding divergent water‐use strategies among encroaching shrubs and the grasses they replace is critical for predicting shifts in ecosystem‐scale water dynamics as a result of shrub encroachment, particularly if drought events become more frequent and/or severe in the future.
In this study, we assessed how water‐use traits of a rapidly encroaching clonal shrub (
Cornus drummondii ) and a dominant C4grass (Andropogon gerardii ) impact responses to changes in water availability in tallgrass prairie. We assessed intra‐annual change in depth of water uptake, turgor loss point and stomatal regulation in each species. Sampling took place at Konza Prairie Biological Station (northeastern KS, USA) during the 2021 and 2022 growing seasons.Cornus drummondii shifted from shallow to deep soil water sources across the 2021 and 2022 growing seasons. This plasticity in depth of water uptake facilitated a ‘wasteful’ water‐use strategy inC. drummondii , where stomatal conductance and transpiration rates continued to increase even when no further gain in photosynthetic rate occurred.A. gerardii photosynthetic rates and stomatal conductance were more variable through time and were more responsive to changes in leaf water potential thanC. drummondii . However, intra‐annual adjustment of turgor loss point was more pronounced inC. drummondii (Δπ TLP = −0.48 MPa ± 0.15 SD) than inA. gerardii (Δπ TLP = −0.29 MPa ± 0.19 SD).Synthesis . These results suggest thatC. drummondii is highly resilient to changes in water availability in surface soils and will likely remain unaffected by future droughts unless they are severe enough to reduce the availability of deep soil water. Given that clonal shrubs are key invaders of grasslands world‐wide, increased leaf‐level water loss is expected to accelerate ecosystem‐level drying as clonal shrub encroachment proceeds in mesic grasslands.Free, publicly-accessible full text available April 1, 2025 -
Abstract Plant populations are limited by resource availability and exhibit physiological trade‐offs in resource acquisition strategies. These trade‐offs may constrain the ability of populations to exhibit fast growth rates under water limitation and high cover of neighbours. However, traits that confer drought tolerance may also confer resistance to competition. It remains unclear how fitness responses to these abiotic conditions and biotic interactions combine to structure grassland communities and how this relationship may change along a gradient of water availability.
To address these knowledge gaps, we estimated the low‐density growth rates of populations in drought conditions with low neighbour cover and in ambient conditions with average neighbour cover for 82 species in six grassland communities across the Central Plains and Southwestern United States. We assessed the relationship between population tolerance to drought and resistance to competition and determined if this relationship was consistent across a precipitation gradient. We also tested whether population growth rates could be predicted using plant functional traits.
Across six sites, we observed a positive correlation between low‐density population growth rates in drought and in the presence of interspecific neighbours. This positive relationship was particularly strong in the grasslands of the northern Great Plains but weak in the most xeric grasslands. High leaf dry matter content and a low (more negative) leaf turgor loss point were associated with high population growth rates in drought and with neighbours in most grassland communities.
Synthesis : A better understanding of how both biotic and abiotic factors impact population fitness provides valuable insights into how grasslands will respond to extreme drought. Our results advance plant strategy theory by suggesting that drought tolerance increases population resistance to interspecific competition in grassland communities. However, this relationship is not evident in the driest grasslands, where above‐ground competition is likely less important. Leaf dry matter content and turgor loss point may help predict which populations will establish and persist based on local water availability and neighbour cover, and these predictions can be used to guide the conservation and restoration of biodiversity in grasslands.Free, publicly-accessible full text available February 1, 2025