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  • Quantification of phenylbutazone in equine plasma for doping control in horse racing using strong anion exchange solid phase extraction followed by liquid chromatography with UV detection
Title: Quantification of phenylbutazone in equine plasma for doping control in horse racing using strong anion exchange solid phase extraction followed by liquid chromatography with UV detection
A method using strong anion exchange solid phase extraction (SAX-SPE) followed by liquid chromatography ultraviolet detection (LC-UV) for the analysis of phenylbutazone (PBZ) and its metabolite oxyphenbutazone (OPBZ) in equine plasma for doping control in horse racing has been developed. By using SAX-SPE, commonly regulated non-steroidal anti-inflammatory drugs (NSAIDs) by the United States Equestrian Federation (USEF), i.e. PBZ, OPBZ, diclofenac, flunixin, ketoprofen, meclofenamic acid and naproxen, and an internal standard, i.e. tolfenamic acid, were first selectively extracted. Then, baseline separation of PBZ, OPBZ from other NSAIDs, internal standard, and residual components of equine plasma was achieved using LC-UV. Finally, PBZ and OPBZ in equine plasma were quantified after an internal calibration curve was created.  more » « less
Award ID(s):
1827209
PAR ID:
10173885
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
111th Illinois State Academy of Science Annual Meeting
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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