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Title: A High-Throughput Microfluidic Magnetic Separation (µFMS) Platform for Water Quality Monitoring
The long-term aim of this work is to develop a biosensing system that rapidly detects bacterial targets of interest, such as Escherichia coli, in drinking and recreational water quality monitoring. For these applications, a standard sample size is 100 mL, which is quite large for magnetic separation microfluidic analysis platforms that typically function with <20 µL/s throughput. Here, we report the use of 1.5-µm-diameter magnetic microdisc to selectively tag target bacteria, and a high-throughput microfluidic device that can potentially isolate the magnetically tagged bacteria from 100 mL water samples in less than 15 min. Simulations and experiments show ~90% capture efficiencies of magnetic particles at flow rates up to 120 µL/s. Also, the platform enables the magnetic microdiscs/bacteria conjugates to be directly imaged, providing a path for quantitative assay.  more » « less
Award ID(s):
1805512 1511953
NSF-PAR ID:
10177452
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
Micromachines
Volume:
11
Issue:
1
ISSN:
2072-666X
Page Range / eLocation ID:
16
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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