Separation of hemodynamic signals from GCaMP fluorescence measured with wide-field imaging
Wide-field calcium imaging is often used to measure brain dynamics in behaving mice. With a large field of view and a high sampling rate, wide-field imaging can monitor activity from several distant cortical areas simultaneously, revealing cortical interactions. Interpretation of wide-field images is complicated, however, by the absorption of light by hemoglobin, which can substantially affect the measured fluorescence. One approach to separating hemodynamics and calcium signals is to use multiwavelength backscatter recordings to measure light absorption by hemoglobin. Following this approach, we develop a spatially detailed regression-based method to estimate hemodynamics. This Spatial Model is based on a linear form of the Beer–Lambert relationship but is fit at every pixel in the image and does not rely on the estimation of physical parameters. In awake mice of three transgenic lines, the Spatial Model offers improved separation of hemodynamics and changes in GCaMP fluorescence. The improvement is pronounced near blood vessels and, in contrast with the Beer–Lambert equations, can remove vascular artifacts along the sagittal midline and in general permits more accurate fluorescence-based determination of neuronal activity across the cortex. NEW & NOTEWORTHY This paper addresses a well-known and strong source of contamination in wide-field calcium-imaging data: hemodynamics. To guide more »
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Publication Date:
NSF-PAR ID:
10309362
Journal Name:
Journal of Neurophysiology
Volume:
123
Issue:
1
ISSN:
0022-3077
2. ABSTRACT This paper presents a detailed analysis of two giant Lyman-alpha (Ly α) arcs detected near galaxies at z = 3.038 and z = 3.754 lensed by the massive cluster MACS 1206−0847 (z = 0.44). The Ly α nebulae revealed in deep MUSE observations exhibit a double-peaked profile with a dominant red peak, indicating expansion/outflowing motions. One of the arcs stretches over 1 arcmin around the cluster Einstein radius, resolving the velocity field of the line-emitting gas on kpc scales around three star-forming galaxies of 0.3–$1.6\, L_*$ at z = 3.038. The second arc spans 15 arcsec in size, roughly centred around two low-mass Ly α emitters ofmore »