More Like this

1. Optical imaging of dental plaque pH

   Chuqin Huang, Manuja Sharma (April 2020, SPIE Medical Imaging 2020: Image-Guided Procedures, Robotic Interventions, and Modeling)

   Tooth decay is one of the most common chronic infectious diseases worldwide. Bacteria from the oral biofilm create a local acidic environment that demineralizes the enamel in the caries disease process. By optically imaging plaque pH in pits and fissures and contacting surfaces of teeth, then medicinal therapies can be accurately applied to prevent or monitor the reversal of caries. To achieve this goal, the fluorescence emission from an aqueous solution of sodium fluorescein was measured using a multimodal scanning fiber endoscope (mmSFE). The 1.6-millimeter diameter mmSFE scans 424nm laser light and collects wide-field reflectance for navigational purposes in grayscale at 30 Hz. Two fluorescence channels centered at 520 and 549 nm are acquired and ratiometric analysis produces a pseudo-color overlay of pH. In vitro measurements calibrate the pH heat maps in the range 4.7 to 7.2 pH (0.2 standard deviation). In vivo measurements of a single case study provides informative images of interproximal biofilm before and after a sugar rinse. Post processing a time series of images provides a method that calculates the average pH changes of oral biofilm, replicating the Stephan Curve. These spatio-temporal records of oral biofilm pH can provide a new method of assessing the risk of tooth decay, guide the application of preventative therapies, and provide a quantitative monitor of overall oral health. The non-contact in vivo optical imaging of pH may be extended to measurements of wound healing, tumor environment, and other food processing surfaces since it relies on low power laser light and a US FDA approved dye. 

   more » « less
2. Machine Learning Approach Identified Multi-Platform Factors for Caries Prediction in Child-Mother Dyads

   https://doi.org/10.3389/fcimb.2021.727630  

   Wu, Tong Tong; Xiao, Jin; Sohn, Michael B.; Fiscella, Kevin A.; Gilbert, Christie; Grier, Alex; Gill, Ann L.; Gill, Steve R. (August 2021, Frontiers in Cellular and Infection Microbiology)

   Untreated tooth decays affect nearly one third of the world and is the most prevalent disease burden among children. The disease progression of tooth decay is multifactorial and involves a prolonged decrease in pH, resulting in the demineralization of tooth surfaces. Bacterial species that are capable of fermenting carbohydrates contribute to the demineralization process by the production of organic acids. The combined use of machine learning and 16s rRNA sequencing offers the potential to predict tooth decay by identifying the bacterial community that is present in an individual’s oral cavity. A few recent studies have demonstrated machine learning predictive modeling using 16s rRNA sequencing of oral samples, but they lack consideration of the multifactorial nature of tooth decay, as well as the role of fungal species within their models. Here, the oral microbiome of mother–child dyads (both healthy and caries-active) was used in combination with demographic–environmental factors and relevant fungal information to create a multifactorial machine learning model based on the LASSO-penalized logistic regression. For the children, not only were several bacterial species found to be caries-associated ( Prevotella histicola, Streptococcus mutans , and Rothia muciloginosa ) but also Candida detection and lower toothbrushing frequency were also caries-associated. Mothers enrolled in this study had a higher detection of S. mutans and Candida and a higher plaque index. This proof-of-concept study demonstrates the significant impact machine learning could have in prevention and diagnostic advancements for tooth decay, as well as the importance of considering fungal and demographic–environmental factors. 

   more » « less
3. Smart Homes as Enablers for Depression Pre-Diagnosis Using PHQ-9 on HMI through Fuzzy Logic Decision System

   https://doi.org/10.3390/s21237864  

   Méndez, Juana Isabel; Meza-Sánchez, Ana Victoria; Ponce, Pedro; McDaniel, Troy; Peffer, Therese; Meier, Alan; Molina, Arturo (December 2021, Sensors)

   Depression is a common mental illness characterized by sadness, lack of interest, or pleasure. According to the DSM-5, there are nine symptoms, from which an individual must present 4 or 5 in the last two weeks to fulfill the diagnosis criteria of depression. Nevertheless, the common methods that health care professionals use to assess and monitor depression symptoms are face-to-face questionnaires leading to time-consuming or expensive methods. On the other hand, smart homes can monitor householders’ health through smart devices such as smartphones, wearables, cameras, or voice assistants connected to the home. Although the depression disorders at smart homes are commonly oriented to the senior sector, depression affects all of us. Therefore, even though an expert needs to diagnose the depression disorder, questionnaires as the PHQ-9 help spot any depressive symptomatology as a pre-diagnosis. Thus, this paper proposes a three-step framework; the first step assesses the nine questions to the end-user through ALEXA or a gamified HMI. Then, a fuzzy logic decision system considers three actions based on the nine responses. Finally, the last step considers these three actions: continue monitoring through Alexa and the HMI, suggest specialist referral, and mandatory specialist referral. 

   more » « less
4. M2G: A Monitor of Monitoring Systems with Ground Truth Validation Features for Research-Oriented Residential Applications

   Ma, C.; Alam, R.; Bell, B.; de la Haye, K.; Spruijt-Metz, D.; Lach, J.; Stankovic, J. (January 2017, MASS)

   Research in the area of internet-of-things, cyber physical- systems, and smart health often employ sensor systems at residences for continuous monitoring. Such research oriented residential monitoring systems (RRMSs) usually face two major challenges, long-term reliable operation management and validation of system functionality with minimal human effort. Targeting these two challenges, this paper describes a monitor of monitoring systems with ground-truth validation capabilities, M2G. It consists of two subsystems, the Monitor2 system and the Ground-truth validation system. The Monitor2 system encapsulates a flexible set of general-purpose components to monitor the operation and connectivity of heterogeneous sensor devices (e.g. smart watches, smart phones, microphones, beacons, etc.), a local base-station, as well as a cloud server. It provides a user-friendly interface and supports different types of RRMSs in various contexts. The system also features a ground truth validation system to support obtaining ground truth in the field. Additionally, customized alerts can be sent to remote administrators and other personnel to report any dysfunction or inaccuracy of the system in real time. M2G is applied to three very different case studies: the M2FED system which monitors family eating dynamics, an in-home wireless sensing system for monitoring nighttime agitation, and the BESI system which monitors behavioral and environmental parameters to predict health events and to provide interventions. The results indicate that M2G is a comprehensive system that (i) requires small cost in time and effort to adapt to an existing RRMS, (ii) provides reliable data collection and reduction in data loss by detecting faults in real-time, and (iii) provides a convenient and timely ground truth validation facility. 

   more » « less
5. Identification of the Bacterial Biosynthetic Gene Clusters of the Oral Microbiome Illuminates the Unexplored Social Language of Bacteria during Health and Disease

   https://doi.org/10.1128/mBio.00321-19  

   Aleti, Gajender; Baker, Jonathon L.; Tang, Xiaoyu; Alvarez, Ruth; Dinis, Márcia; Tran, Nini C.; Melnik, Alexey V.; Zhong, Cuncong; Ernst, Madeleine; Dorrestein, Pieter C.; et al (April 2019, mBio)

   ABSTRACT Small molecules are the primary communication media of the microbial world. Recent bioinformatic studies, exploring the biosynthetic gene clusters (BGCs) which produce many small molecules, have highlighted the incredible biochemical potential of the signaling molecules encoded by the human microbiome. Thus far, most research efforts have focused on understanding the social language of the gut microbiome, leaving crucial signaling molecules produced by oral bacteria and their connection to health versus disease in need of investigation. In this study, a total of 4,915 BGCs were identified across 461 genomes representing a broad taxonomic diversity of oral bacteria. Sequence similarity networking provided a putative product class for more than 100 unclassified novel BGCs. The newly identified BGCs were cross-referenced against 254 metagenomes and metatranscriptomes derived from individuals either with good oral health or with dental caries or periodontitis. This analysis revealed 2,473 BGCs, which were differentially represented across the oral microbiomes associated with health versus disease. Coabundance network analysis identified numerous inverse correlations between BGCs and specific oral taxa. These correlations were present in healthy individuals but greatly reduced in individuals with dental caries, which may suggest a defect in colonization resistance. Finally, corroborating mass spectrometry identified several compounds with homology to products of the predicted BGC classes. Together, these findings greatly expand the number of known biosynthetic pathways present in the oral microbiome and provide an atlas for experimental characterization of these abundant, yet poorly understood, molecules and socio-chemical relationships, which impact the development of caries and periodontitis, two of the world’s most common chronic diseases. IMPORTANCE The healthy oral microbiome is symbiotic with the human host, importantly providing colonization resistance against potential pathogens. Dental caries and periodontitis are two of the world’s most common and costly chronic infectious diseases and are caused by a localized dysbiosis of the oral microbiome. Bacterially produced small molecules, often encoded by BGCs, are the primary communication media of bacterial communities and play a crucial, yet largely unknown, role in the transition from health to dysbiosis. This study provides a comprehensive mapping of the BGC repertoire of the human oral microbiome and identifies major differences in health compared to disease. Furthermore, BGC representation and expression is linked to the abundance of particular oral bacterial taxa in health versus dental caries and periodontitis. Overall, this study provides a significant insight into the chemical communication network of the healthy oral microbiome and how it devolves in the case of two prominent diseases. 

   more » « less