More Like this

1. Enhancing Quantum Emission from Spin Defects in Hexagonal Boron Nitride with a Plasmonic Nanocavity

   https://doi.org/10.1364/CLEO_FS.2023.FTh3A.1  

   Xu, Xiaohui ; Solanki, Abhishek. B. ; Sychev, Demid ; Gao, Xingyu ; Peana, Samuel ; Baburin, Alexander S. ; Pagadala, Karthik ; Martin, Zachariah O. ; Chowdhury, Sarah N. ; Chen, Yong P. ; et al ( January 2023 , Enhancing Quantum Emission from Spin Defects in Hexagonal Boron Nitride with a Plasmonic Nanocavity)

   We report a 250-fold photoluminescence enhancement of V_B^-spin-defects in hBN by coupling them to nanopatch antennas (NPA). Considering the relative size of the NPAs and laser-spot, an actual enhancement of 1695 times is determined.

    

   more » « less
2. Photoacoustic Imaging Quantifies Drug Release from Nanocarriers via Redox Chemistry of Dye‐Labeled Cargo

   https://doi.org/10.1002/anie.201914120  

   Jeevarathinam, Ananthakrishnan Soundaram ; Lemaster, Jeanne E. ; Chen, Fang ; Zhao, Eric ; Jokerst, Jesse V. ( January 2020 , Angewandte Chemie International Edition)

   We report a new approach to monitor drug release from nanocarriers via a paclitaxel–methylene blue conjugate (PTX‐MB) with redox activity. This construct is in a photoacoustically silent reduced state inside poly(lactic‐co‐glycolic acid) (PLGA) nanoparticles (PTX‐MB@PLGA NPs). During release, PTX‐MB is spontaneously oxidized to produce a concentration‐dependent photoacoustic signal. An in vitro drug‐release study showed an initial burst release (25 %) between 0–24 h and a sustained release between 24–120 h with a cumulative release of 40.6 % and a 670‐fold increase in photoacoustic signal. An in vivo murine drug release showed a photoacoustic signal enhancement of up to 649 % after 10 hours. PTX‐MB@PLGA NPs showed an IC₅₀of 78 μg mL⁻¹and 44.7±4.8 % decrease of tumor burden in an orthotopic model of colon cancer via luciferase‐positive CT26 cells.

    

   more » « less
3. Photoacoustic Imaging Quantifies Drug Release from Nanocarriers via Redox Chemistry of Dye‐Labeled Cargo

   https://doi.org/10.1002/ange.201914120  

   Jeevarathinam, Ananthakrishnan Soundaram ; Lemaster, Jeanne E. ; Chen, Fang ; Zhao, Eric ; Jokerst, Jesse V. ( January 2020 , Angewandte Chemie)

   We report a new approach to monitor drug release from nanocarriers via a paclitaxel–methylene blue conjugate (PTX‐MB) with redox activity. This construct is in a photoacoustically silent reduced state inside poly(lactic‐co‐glycolic acid) (PLGA) nanoparticles (PTX‐MB@PLGA NPs). During release, PTX‐MB is spontaneously oxidized to produce a concentration‐dependent photoacoustic signal. An in vitro drug‐release study showed an initial burst release (25 %) between 0–24 h and a sustained release between 24–120 h with a cumulative release of 40.6 % and a 670‐fold increase in photoacoustic signal. An in vivo murine drug release showed a photoacoustic signal enhancement of up to 649 % after 10 hours. PTX‐MB@PLGA NPs showed an IC₅₀of 78 μg mL⁻¹and 44.7±4.8 % decrease of tumor burden in an orthotopic model of colon cancer via luciferase‐positive CT26 cells.

    

   more » « less
4. A Photoacoustic‐Fluorescent Imaging Probe for Proteolytic Gingipains Expressed by Porphyromonas gingivalis

   https://doi.org/10.1002/ange.202201843  

   Moore, Colman ; Cheng, Yong ; Tjokro, Natalia ; Zhang, Brendan ; Kerr, Matthew ; Hayati, Mohammed ; Chang, Kai Chiao Joe ; Shah, Nisarg ; Chen, Casey ; Jokerst, Jesse V. ( June 2022 , Angewandte Chemie)

   Porphyromonas gingivalisis a keystone pathogen in periodontal disease. We herein report a dual‐modal fluorescent and photoacoustic imaging probe for the detection of gingipain proteases secreted byP. gingivalis. Upon proteolytic cleavage by Arg‐specific gingipain (RgpB), five‐fold photoacoustic enhancement and >100‐fold fluorescence activation was measured with detection limits of 1.1 nM RgpB and 5.0E4 CFU mL⁻¹bacteria in vitro. RgpB activity was imaged in porcine jaws with low‐nanomolar sensitivity. Diagnostic efficacy was evaluated in gingival crevicular fluid samples from subjects with and without periodontal disease, wherein activation was correlated to qPCR‐based detection ofP. gingivalis(Pearson'sr=0.71). Finally, photoacoustic imaging of RgpB‐cleaved probe was achieved in murine brains ex vivo, with relevance and potential utility for disease models of general infection byP. gingivalis, motivated by the recent biological link between gingipain and Alzheimer's disease.

    

   more » « less
5. A Photoacoustic‐Fluorescent Imaging Probe for Proteolytic Gingipains Expressed by Porphyromonas gingivalis

   https://doi.org/10.1002/anie.202201843  

   Moore, Colman ; Cheng, Yong ; Tjokro, Natalia ; Zhang, Brendan ; Kerr, Matthew ; Hayati, Mohammed ; Chang, Kai Chiao Joe ; Shah, Nisarg ; Chen, Casey ; Jokerst, Jesse V. ( June 2022 , Angewandte Chemie International Edition)

   Porphyromonas gingivalisis a keystone pathogen in periodontal disease. We herein report a dual‐modal fluorescent and photoacoustic imaging probe for the detection of gingipain proteases secreted byP. gingivalis. Upon proteolytic cleavage by Arg‐specific gingipain (RgpB), five‐fold photoacoustic enhancement and >100‐fold fluorescence activation was measured with detection limits of 1.1 nM RgpB and 5.0E4 CFU mL⁻¹bacteria in vitro. RgpB activity was imaged in porcine jaws with low‐nanomolar sensitivity. Diagnostic efficacy was evaluated in gingival crevicular fluid samples from subjects with and without periodontal disease, wherein activation was correlated to qPCR‐based detection ofP. gingivalis(Pearson'sr=0.71). Finally, photoacoustic imaging of RgpB‐cleaved probe was achieved in murine brains ex vivo, with relevance and potential utility for disease models of general infection byP. gingivalis, motivated by the recent biological link between gingipain and Alzheimer's disease.

    

   more » « less