Pressure is important in virtually all problems in fluid dynamics from macro-scale to micro/nano-scale flows. Although technologies are well developed for its measurement at the macroscopic scale, pressure quantification at the microscopic scale is still not trivial. This study reports the design and fabrication of an on-chip sensor that enables quantification of pressure in microfluidic devices based on a novel technique called astigmatic particle tracking. With this technique, thin membranes that sense pressure variations in the fluid flow can be characterized conveniently by imaging the shapes of the particles embedded in the membranes. This innovative design only relies on the reflected light from the back of the microchannel, rendering the sensor to be separate and noninvasive to the flow of interest. This sensor was then applied to characterize the pressure drop in single-phase flows with an accuracy of ∼70 Pa and good agreement was achieved between the sensor, a commercial pressure transducer and numerical simulation results. Additionally, the sensor successfully measured the capillary pressure across an air–water interface with a 7% deviation from the theoretical value. To the best of our knowledge, this pore-scale capillary pressure quantification is achieved for the first time using an on-chip pressure sensor of this kind. This study provides a novel method for in situ quantification of local pressure and thus opens the door to a renewed understanding of pore-scale physics of local pressure in multi-phase flow in porous media.
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Microfluidic pressure in paper (μPiP): rapid prototyping and low-cost liquid handling for on-chip diagnostics
Paper-based microfluidics was initially developed for use in ultra-low-cost diagnostics powered passively by liquid wicking. However, there is significant untapped potential in using paper to internally guide porous microfluidic flows using externally applied pressure gradients. Here, we present a new technique for fabricating and utilizing low-cost polymer-laminated paper-based microfluidic devices using external pressure. Known as microfluidic pressure in paper (μPiP), devices fabricated by this technique are capable of sustaining a pressure gradient for use in precise liquid handling and manipulation applications similar to conventional microfluidic open-channel designs, but instead where fluid is driven directly through the porous paper structure. μPiP devices can be both rapidly prototyped or scalably manufactured and deployed at commercial scale with minimal time, equipment, and training requirements. We present an analysis of continuous pressure-driven flow in porous paper-based microfluidic channels and demonstrate broad applicability of this method in performing a variety of different liquid handling applications, including measuring red blood cell deformability and performing continuous free-flow DNA electrophoresis. This new platform offers a budget-friendly method for performing microfluidic operations for both academic prototyping and large-scale commercial device production.
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- Award ID(s):
- 2112224
- NSF-PAR ID:
- 10349991
- Date Published:
- Journal Name:
- The Analyst
- Volume:
- 147
- Issue:
- 4
- ISSN:
- 0003-2654
- Page Range / eLocation ID:
- 587 to 596
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1039/D1AN01676H
