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Abstract Nanocarrier and exosome encapsulation has been found to significantly increase the efficacy of targeted drug delivery while also minimizing unwanted side effects. However, the development of exosome-encapsulated drug nanocarriers is limited by low drug loading efficiencies and/or complex, time-consuming drug loading processes. Herein, we have developed an acoustofluidic device that simultaneously performs both drug loading and exosome encapsulation. By synergistically leveraging the acoustic radiation force, acoustic microstreaming, and shear stresses in a rotating droplet, the concentration, and fusion of exosomes, drugs, and porous silica nanoparticles is achieved. The final product consists of drug-loaded silica nanocarriers that are encased within an exosomal membrane. The drug loading efficiency is significantly improved, with nearly 30% of the free drug (e.g., doxorubicin) molecules loaded into the nanocarriers. Furthermore, this acoustofluidic drug loading system circumvents the need for complex chemical modification, allowing drug loading and encapsulation to be completed within a matter of minutes. These exosome-encapsulated nanocarriers exhibit excellent efficiency in intracellular transport and are capable of significantly inhibiting tumor cell proliferation. By utilizing physical forces to rapidly generate hybrid nanocarriers, this acoustofluidic drug loading platform wields the potential to significantly impact innovation in both drug delivery research and applications.
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Engineering endosomolytic nanocarriers of diverse morphologies using confined impingement jet mixing
Confined impingement jet (CIJ) mixing was utilized to fabricate pH-responsive endosomolytic polymeric nanocarriers. Manipulation of polymer and formulation properties facilitated the production of multiple nanocarriers with distinct characteristics.
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- Award ID(s):
- 1852157
- PAR ID:
- 10480921
- Publisher / Repository:
- RSC
- Date Published:
- Journal Name:
- Nanoscale
- Volume:
- 15
- Issue:
- 39
- ISSN:
- 2040-3364
- Page Range / eLocation ID:
- 16016 to 16029
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1039/D3NR02874G
