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Title: Development of Gelatin-Coated Hydrogel Microspheres for Novel Bioink Design: A Crosslinker Study
The development of vascularized tissue is a substantial challenge within the field of tissue engineering and regenerative medicine. Studies have shown that positively-charged microspheres exhibit dual-functions: (1) facilitation of vascularization and (2) controlled release of bioactive compounds. In this study, gelatin-coated microspheres were produced and processed with either EDC or transglutaminase, two crosslinkers. The results indicated that the processing stages did not significantly impact the size of the microspheres. EDC and transglutaminase had different effects on surface morphology and microsphere stability in a simulated colonic environment. Incorporation of EGM and TGM into bioink did not negatively impact bioprintability (as indicated by density and kinematic viscosity), and the microspheres had a uniform distribution within the scaffold. These microspheres show great potential for tissue engineering applications.  more » « less
Award ID(s):
2045738
PAR ID:
10494463
Author(s) / Creator(s):
; ; ; ; ; ; ; ; ; ;
Corporate Creator(s):
Editor(s):
Guy Van der Mooter
Publisher / Repository:
Pharmaceutics
Date Published:
Journal Name:
Pharmaceutics
Volume:
15
Issue:
1
ISSN:
1999-4923
Page Range / eLocation ID:
90
Subject(s) / Keyword(s):
pectin hydrogel electrospray gelatin microspheres bioink vascularization
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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