skip to main content
US FlagAn official website of the United States government
dot gov icon
Official websites use .gov
A .gov website belongs to an official government organization in the United States.
https lock icon
Secure .gov websites use HTTPS
A lock ( lock ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

Attention:

The NSF Public Access Repository (PAR) system and access will be unavailable from 11:00 PM ET on Thursday, June 12 until 2:00 AM ET on Friday, June 13 due to maintenance. We apologize for the inconvenience.

Explore Research Products in the PAR
It may take a few hours for recently added research products to appear in PAR search results.


  • NSF Public Access
  • Search Results
  • Impact of the coffee berry borer on the volatile and semi-volatile compounds; qualitative profile of Coffea arabica berries
Title: Impact of the coffee berry borer on the volatile and semi-volatile compounds; qualitative profile of Coffea arabica berries
One of the main attributes that highlight the final quality of a gourmet cup of coffee is its aroma. Aromas vary according to a variety of plant and environmental variables, among others. This study aimed to characterize volatile and semivolatile compounds according to the Coffee arabica "Limani" berries ripening stages (healthy and brocaded). The study used different extraction methodologies to capture the broad spectrum of volatile, semivolatile organic compounds in coffee berries and berry borer (CBB). The methodologies used in the study included: enfleurage, headspace SPME (solid-phase microextraction), absorbent trap, and direct immersion SPME. Our study generated a Profile for coffee berries and CBB w with 228 compounds. Esters, cyclic, and benzyl compounds represent 65.6% of the total. The first three types of compounds that most attract our sense of smell constitute 40.5% of the compounds found; 1.3% aldehydes, 2.6% alcohols, and 36.6% benzyl. Overripe berries have high volatile emissions and show a composition dominated mainly by esters followed by alcohols, ketones, and aldehydes. The lowest-level compounds were monoterpenes. The number of compounds found in CBB varied according to sex. In summary, the CBB damage harms coffee berries' quality and aroma. The complete profile compounds generated will help better understand insect-plant relationships and potentially develop effective bait traps.  more » « less
Award ID(s):
1736093
PAR ID:
10497561
Author(s) / Creator(s):
; ; ;
Publisher / Repository:
Elsevier
Date Published:
Journal Name:
Food Chemistry Advances
Volume:
2
Issue:
C
ISSN:
2772-753X
Page Range / eLocation ID:
100154
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
More Like this
  1. ; ; ; (, Microplastics)
    Microplastics are tangible particles of less than 0.2 inches in diameter that are ubiquitously distributed in the biosphere and accumulate in water bodies. During the east-coast hot summers (23–29 °C) of 2021 and 2022, June through September, we captured copious amounts of the jellyfish Chrysaora chesapeakei, a predominant species found in the Patuxent River of the Chesapeake Bay in Maryland on the United States East Coast. We determined that their gelatinous bodies trapped many microplastics through fluorescent microscopy studies using Rhodamine B staining and Raman Spectroscopy. The chemical nature of the microplastics was detected using gas chromatography–mass spectroscopy headspace (SPME-GC-MS) and solvent extraction (GC-MS) methods through a professional commercial materials evaluation laboratory. Numerous plastic-affiliated volatile organic compounds (VOCs) from diverse chemical origins and their functional groups (alkanes, alkenes, acids, aldehydes, ketones, ethers, esters, and alcohols) along with other non-microplastic volatile organic compounds were observed. Our findings corroborate data in the available scientific literature, distinguishing our finding’s suitability. 
    more » « less
  2. ; ; ; (, Journal of Forensic Sciences)
    Abstract Volatile organic compounds (VOCs) are by‐products of cadaveric decomposition and are responsible for the odor associated with decomposing remains. The direct link betweenVOCproduction and individual postmortem microbes has not been well characterized experimentally. The purpose of this study was to profileVOCs released from three postmortem bacterial isolates (Bacillus subtilis, Ignatzschineria indica, I. ureiclastica)using solid‐phase microextraction arrow (SPMEArrow) and gas chromatography–mass spectrometry (GC‐MS). Species were inoculated in headspace vials on Standard Nutrient Agar and monitored over 5 days at 24°C. Each species exhibited a differentVOCprofile that included common decompositionVOCs.VOCs exhibited upward or downward temporal trends over time.Ignatzschineria indicaproduced a large amount of dimethyldisulfide. Other compounds of interest included alcohols, aldehydes, aromatics, and ketones. This provides foundational data to link decomposition odor with specific postmortem microbes to improve understanding of underlying mechanisms for decompositionVOCproduction. 
    more » « less
  3. ; (, Ecological Applications)
    Abstract Natural pest control is an alternative to pesticide use in agriculture, and may help to curb insect declines and promote crop production. Nonconsumptive interactions in natural pest control that historically have received far less attention than consumptive interactions, may have distinct impacts on pest damage suppression and may also mediate positive multipredator interactions. Additionally, when nonconsumptive effects are driven by natural enemy aggression, variation in alternative resources for enemies may impact the strength of pest control. Here we study control of the coffee berry borer (CBB),Hypothenemus hampei, by a keystone arboreal ant species,Azteca sericeasur, which exhibits a nonconsumptive effect on CBB by throwing them off coffee plants. We conducted two experiments to investigate: (1) if the strength of this behavior is driven by spatial or temporal variability in scale insect density (an alternative resource thatAztecatends for honeydew), (2) if this behavior mediates positive interactions betweenAztecaand other ground‐foraging ants, and (3) the effect this behavior has on the overall suppression of CBB damage in multipredator scenarios. Our behavioral experiment showed that nearly all interactions betweenAztecaand CBB are nonconsumptive and that this behavior occurs more frequently in the dry season and with higher densities of scale insects on coffee branches. Our multipredator experiment revealed that borers thrown off coffee plants byAztecacan survive and potentially damage other nearby plants but may be suppressed by ground‐foraging ants. Although we found no non‐additive effects betweenAztecaand ground‐foraging ants on overall CBB damage, together, both species resulted in the lowest level of plant damage with the subsequent reduction in “spillover” damage caused by thrown CBB, indicating spatial complementarity between predators. These results present a unique case of natural pest control, in which damage suppression is driven almost exclusively by nonconsumptive natural enemy aggression, as opposed to consumption or prey behavioral changes. Furthermore, our results demonstrate the variability that may occur in nonconsumptive pest control interactions when natural enemy aggressive behavior is impacted by alternative resources, and also show how these nonconsumptive effects can mediate positive interactions between natural enemies to enhance overall crop damage reduction. 
    more » « less
  4. ; ; ; ; ; ; ; ; ; ; et al (, Atmospheric Chemistry and Physics)
    Volatile chemical products (VCPs) and other non-combustion-related sourceshave become important for urban air quality, and bottom-up calculationsreport emissions of a variety of functionalized compounds that remainunderstudied and uncertain in emissions estimates. Using a new instrumentalconfiguration, we present online measurements of oxygenated organiccompounds in a US megacity over a 10 d wintertime sampling period, whenbiogenic sources and photochemistry were less active. Measurements wereconducted at a rooftop observatory in upper Manhattan, New York City, USAusing a Vocus chemical ionization time-of-flight mass spectrometer, withammonium (NH4+) as the reagent ion operating at 1 Hz. The range ofobservations spanned volatile, intermediate-volatility, and semi-volatileorganic compounds, with targeted analyses of ∼150 ions, whoselikely assignments included a range of functionalized compound classes suchas glycols, glycol ethers, acetates, acids, alcohols, acrylates, esters,ethanolamines, and ketones that are found in various consumer, commercial,and industrial products. Their concentrations varied as a function of winddirection, with enhancements over the highly populated areas of the Bronx,Manhattan, and parts of New Jersey, and included abundant concentrations ofacetates, acrylates, ethylene glycol, and other commonly used oxygenatedcompounds. The results provide top-down constraints on wintertime emissionsof these oxygenated and functionalized compounds, with ratios to commonanthropogenic marker compounds and comparisons of their relative abundancesto two regionally resolved emissions inventories used in urban air qualitymodels. 
    more » « less
  5. ; ; ; ; ; ; (, Journal of Chromatography A)
    Decomposition odor is produced during postmortem mammalian tissue breakdown by bacteria, insects, and intrinsic chemical processes. Past research has not thoroughly investigated which volatile organic compounds (VOCs) can be linked directly to individual bacterial species on decomposing remains. The purpose of this study was to profile the VOCs produced over time by individual species of bacteria using comprehensive two- dimensional gas chromatography (GC×GC) to expand our foundational knowledge of what each bacterial spe­cies contributes to decomposition odor. Five different species of bacteria (Bacillus subtilis, Ignatzschineria indica, Ignatzschineria ureiclastica, Curtobacterium luteum, and Vagococcus lutrae) were cultured on standard nutrient agar individually and monitored daily using solid phase microextraction arrow (SPME Arrow) and GC×GC in combination with quadrupole mass spectrometry (qMS) and flame ionization detection (FID). The GC×GC-qMS/FID approach was used to generate rich VOC profiles that represented the bacterial species’ metabolic VOC pro­ duction longitudinally. The data obtained from the chromatographic output was used to compare with a prior study using one-dimensional GC-qMS, and also between each of the five species to investigate the extent of overlap between species. No single VOC could be found in all five bacterial species investigated, and there was little overlap in the profile between species. To further visualize these differences, chromatographic peak data was investigated using two different ordination strategies, principal component analysis (PCA) and principal coordinate analysis (PCoA). The two ordination strategies were compared with each other using a Procrustes analysis. This was performed to understand differences in ordination strategies between the separation science community and chemical ecological community. Overall, ordination strategies were found to produce similar results, as evidenced by the correlation of PCA and PCoA in the Procrustes analysis. All analysis strategies yielded distinct VOC profiles for each species. Further study of additional species will support understanding of the holistic view of decomposition odor from a chemical ecology perspective, and further support our understanding of the production of decomposition odor that culminates from such a complex environment. 
    more » « less
  • Citation Formats
  • MLA
  • APA
  • Chicago
  • BibTeX
  • Save / Share this Record
  • Send to Email