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Title: Protocol for fixing and peeling Aedes aegypti embryos v1
The collection, fixing, and immunohistochemical staining of Aedes aegypti embryos is challenging in comparison to D. melanogaster since the vitelline membrane of Ae. aegypti must be manually removed. Herein we report on an improvement for the methods to prepare Ae. aegypti embryos. The adapted protocol increases the throughput capacity of embryos by an individual user, with experienced users able to process an average of 100-150 embryos per hour. The protocol provides high-quality intact embryos that can be used for morphological, immunohistochemical, and in situ RNA hybridization studies. Critical to the success of the optimized protocol is the selection and description of the tools required, and differing approaches for younger and older embryos.  more » « less
Award ID(s):
1911723
PAR ID:
10539208
Author(s) / Creator(s):
; ;
Publisher / Repository:
protocols.io
Date Published:
Format(s):
Medium: X
Institution:
University at Buffalo-State University of New York
Sponsoring Org:
National Science Foundation
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