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In Drosophila melanogaster genetic screens are often used to identify genes associated with different biological processes. Here, we have utilized the Flp/FRT system to generate mitotic clones within the developing eye. These clones were screened for mutations that disrupt cell division, organ patterning, and cell growth. One such mutation from this screen, mutant M.3.2, resulted in an expansion of the cuticle within the area normally covered by ommatidium as well as an overall smaller eye size. Genetic and molecular mapping revealed this mutation to be in the gene, tout-velu (ttv).
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G.3.2 is a novel allele of the gene connector enhancer of ksr (cnk) in Drosophila melanogaster
Genetic screens in Drosophila melanogaster have long been used to identify genes found in a variety of developmental, cellular, and behavioral processes. Here we describe the characterization and mapping of a mutation identified in a conditional screen for genetic regulators of cell growth and cell division. Within a Flp/FRT system, mutant G.3.2 results in a reduction of mutant tissue and a rough eye phenotype. We find that G.3.2 maps to the gene cnk, providing further support that cnk is a critical gene in Drosophila eye development. This mutant was characterized, mapped and sequenced by undergraduate students within the Fly-CURE consortium.
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- Award ID(s):
- 2316218
- PAR ID:
- 10630847
- Author(s) / Creator(s):
- ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; more »
- Publisher / Repository:
- microPublication Biology
- Date Published:
- Journal Name:
- microPublication biology
- Volume:
- 2024
- ISSN:
- 2578-9430
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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The mutation I.3.2 was previously identified in a FLP/FRT screen of chromosome 2R for conditional growth regulators. Here we report the phenotypic characterization and genetic mapping of I.3.2 by undergraduate students participating in Fly-CURE, a pedagogical program that teaches the science of genetics through a classroom research experience. We find that creation of I.3.2 cell clones in the developing eye-antennal imaginal disc causes a headless adult phenotype, suggestive of both autonomous and non-autonomous effects on cell growth or viability. We also identify the I.3.2 mutation as a loss-of-function allele of the gene centromere identifier (cid), which encodes centromere-specific histone H3 variant critical for chromosomal segregation.more » « less
- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.17912/micropub.biology.001290
