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Abstract Motivation Sketching is now widely used in bioinformatics to reduce data size and increase data processing speed. Sketching approaches entice with improved scalability but also carry the danger of decreased accuracy and added bias. In this article, we investigate the minimizer sketch and its use to estimate the Jaccard similarity between two sequences. Results We show that the minimizer Jaccard estimator is biased and inconsistent, which means that the expected difference (i.e. the bias) between the estimator and the true value is not zero, even in the limit as the lengths of the sequences grow. We derive an analytical formula for the bias as a function of how the shared k-mers are laid out along the sequences. We show both theoretically and empirically that there are families of sequences where the bias can be substantial (e.g. the true Jaccard can be more than double the estimate). Finally, we demonstrate that this bias affects the accuracy of the widely used mashmap read mapping tool. Availability and implementation Scripts to reproduce our experiments are available at https://github.com/medvedevgroup/minimizer-jaccard-estimator/tree/main/reproduce. Supplementary information Supplementary data are available at Bioinformatics online. 

Abstract Galaxy is a mature, browser accessible workbench for scientific computing. It enables scientists to share, analyze and visualize their own data, with minimal technical impediments. A thriving global community continues to use, maintain and contribute to the project, with support from multiple national infrastructure providers that enable freely accessible analysis and training services. The Galaxy Training Network supports free, self-directed, virtual training with >230 integrated tutorials. Project engagement metrics have continued to grow over the last 2 years, including source code contributions, publications, software packages wrapped as tools, registered users and their daily analysis jobs, and new independent specialized servers. Key Galaxy technical developments include an improved user interface for launching large-scale analyses with many files, interactive tools for exploratory data analysis, and a complete suite of machine learning tools. Important scientific developments enabled by Galaxy include Vertebrate Genome Project (VGP) assembly workflows and global SARS-CoV-2 collaborations. 

Compute heterogeneity is increasingly gaining prominence in modern datacenters due to the addition of accelerators like GPUs and FPGAs. We observe that datacenter schedulers are agnostic of these emerging accelerators, especially their resource utilization footprints, and thus, not well equipped to dynamically provision them based on the application needs. We observe that the state-of-the-art datacenter schedulers fail to provide fine-grained resource guarantees for latency-sensitive tasks that are GPU-bound. Specifically for GPUs, this results in resource fragmentation and interference leading to poor utilization of allocated GPU resources. Furthermore, GPUs exhibit highly linear energy efficiency with respect to utilization and hence proactive management of these resources is essential to keep the operational costs low while ensuring the end-to-end Quality of Service (QoS) in case of user-facing queries.Towards addressing the GPU orchestration problem, we build Knots, a GPU-aware resource orchestration layer and integrate it with the Kubernetes container orchestrator to build Kube- Knots. Kube-Knots can dynamically harvest spare compute cycles through dynamic container orchestration enabling co-location of latency-critical and batch workloads together while improving the overall resource utilization. We design and evaluate two GPU-based scheduling techniques to schedule datacenter-scale workloads through Kube-Knots on a ten node GPU cluster. Our proposed Correlation Based Prediction (CBP) and Peak Prediction (PP) schemes together improves both average and 99 th percentile cluster-wide GPU utilization by up to 80% in case of HPC workloads. In addition, CBP+PP improves the average job completion times (JCT) of deep learning workloads by up to 36% when compared to state-of-the-art schedulers. This leads to 33% cluster-wide energy savings on an average for three different workloads compared to state-of-the-art GPU-agnostic schedulers. Further, the proposed PP scheduler guarantees the end-to-end QoS for latency-critical queries by reducing QoS violations by up to 53% when compared to state-of-the-art GPU schedulers.